rabbit polyclonal anti leptin  (Bioss)

Journal: Frontiers in Immunology

Article Title: Leptin deficiency in CD8 + T cells ameliorates non-segmental vitiligo by reducing interferon-γ and Granzyme B

doi: 10.3389/fimmu.2023.1158883

Figure Lengend Snippet: LEPR expression in CD8 + T cells from vitiligo patients was increased. (A, B) Double immunofluorescence staining results of LEPR and CD8 in vitiligo lesions and normal skin (Vitiligo: N = 5, NC: N = 5). (A) CD8 was represented by green fluorescence and LEPR was represented by red fluorescence. (B) The expression of LEPR in CD8 + T cells in Vitiligo lesions was significantly higher. (C) The serum levels of leptin were evaluated by ELISA (Vitiligo: N=53, HC: N=36). (D, E) Representative flow cytometric profiles and data plots of CD8 + T cells in PBMCs are shown (Vitiligo: N=5, HC: N=5). * P < 0.05, ** P < 0.01.

Article Snippet: The following antibodies were used: Alexa Flour 647 Donkey anti-rabbit IgG (BioLegend), Rabbit Anti-Leptin receptor antibody (bs-0410R, Bioss), APC-Cy7 anti-human CD4 (BD Biosciences), Percp-Cy5.5 anti-human CD8 (BD Biosciences), PE anti-human Perforin (BD Biosciences), FITC anti-human/mouse Granzyme B (Granzyme B, BioLegend), PE-Cy7 anti-human IFN-γ (BioLegend), Zombie Aqua Fixable Viability Kit (BioLegend), APC-Cy7 anti-mouse CD4 (Abcam), Percp-Cy5.5 anti-mouse CD8 (Abcam), PE anti-mouse Perforin (Abcam), FITC anti-mouse/human granzyme B (BioLegend), PE-Cy7 anti-mouse IFN-γ (Abcam).

Techniques: Expressing, Double Immunofluorescence Staining, Fluorescence, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Leptin deficiency in CD8 + T cells ameliorates non-segmental vitiligo by reducing interferon-γ and Granzyme B

doi: 10.3389/fimmu.2023.1158883

Figure Lengend Snippet: Leptin enhances the expression of cytotoxic cytokines from CD8 + T cells in vitro . (A, B) Gating strategy, representative flow cytometric plots, and statistical analysis of the percentage of cell subsets were shown. (C) After 72 hours of leptin stimulation, the protein levels of IFN-γ, perforin, and Granzyme B from normal PBMCs were detected by ELISA. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The following antibodies were used: Alexa Flour 647 Donkey anti-rabbit IgG (BioLegend), Rabbit Anti-Leptin receptor antibody (bs-0410R, Bioss), APC-Cy7 anti-human CD4 (BD Biosciences), Percp-Cy5.5 anti-human CD8 (BD Biosciences), PE anti-human Perforin (BD Biosciences), FITC anti-human/mouse Granzyme B (Granzyme B, BioLegend), PE-Cy7 anti-human IFN-γ (BioLegend), Zombie Aqua Fixable Viability Kit (BioLegend), APC-Cy7 anti-mouse CD4 (Abcam), Percp-Cy5.5 anti-mouse CD8 (Abcam), PE anti-mouse Perforin (Abcam), FITC anti-mouse/human granzyme B (BioLegend), PE-Cy7 anti-mouse IFN-γ (Abcam).

Techniques: Expressing, In Vitro, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Leptin deficiency in CD8 + T cells ameliorates non-segmental vitiligo by reducing interferon-γ and Granzyme B

doi: 10.3389/fimmu.2023.1158883

Figure Lengend Snippet: Leptin deficiency in CD8 + T cells ameliorated vitiligo development in mice. (A) The hair decolorization areas of mice with vitiligo induced by monobenzone are shown, marked with red circles. (B) HE staining showed that the inflammatory cells of the Lep KO mice were significantly reduced compared with the WT group. (C) The heatmap shows the differential expression of vitiligo-related genes between WT and Lep KO mice after monobenzone was administrated. (D) RT-qPCR was used to verify the differentially expressed genes in CD8 + T cells from spleens of vitiligo mice induced by monobenzone in the Lep KO group and WT group. * P < 0.05, *** P < 0.001.

Article Snippet: The following antibodies were used: Alexa Flour 647 Donkey anti-rabbit IgG (BioLegend), Rabbit Anti-Leptin receptor antibody (bs-0410R, Bioss), APC-Cy7 anti-human CD4 (BD Biosciences), Percp-Cy5.5 anti-human CD8 (BD Biosciences), PE anti-human Perforin (BD Biosciences), FITC anti-human/mouse Granzyme B (Granzyme B, BioLegend), PE-Cy7 anti-human IFN-γ (BioLegend), Zombie Aqua Fixable Viability Kit (BioLegend), APC-Cy7 anti-mouse CD4 (Abcam), Percp-Cy5.5 anti-mouse CD8 (Abcam), PE anti-mouse Perforin (Abcam), FITC anti-mouse/human granzyme B (BioLegend), PE-Cy7 anti-mouse IFN-γ (Abcam).

Techniques: Staining, Expressing, Quantitative RT-PCR